md 2 Search Results


recombinant human his md2  (R&D Systems)
94
R&D Systems recombinant human his md2
Recombinant Human His Md2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human his md2/product/R&D Systems
Average 94 stars, based on 1 article reviews
recombinant human his md2 - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
control monoclonal mouse igg  (R&D Systems)
92
R&D Systems control monoclonal mouse igg
Control Monoclonal Mouse Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/control monoclonal mouse igg/product/R&D Systems
Average 92 stars, based on 1 article reviews
control monoclonal mouse igg - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
tlr4 md 2 complex  (R&D Systems)
93
R&D Systems tlr4 md 2 complex
Figure 6. Potential roles of the DNA-mediated proteolytic processing of HMGB1 by neutrophil elastase in NETs. Due to the enhanced binding activities of the processed HMGB1 protein, this processing may promote (1) <t>TLR4</t> signaling, (2) binding to biofilm DNA, and (3) DNA sensing by cGAS. Due to the loss of residues 177–215, the processing of HMGB1 may diminish (4) RAGE signaling and (5) nuclear localization. NET, neutrophil extracellular trap.
Tlr4 Md 2 Complex, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tlr4 md 2 complex/product/R&D Systems
Average 93 stars, based on 1 article reviews
tlr4 md 2 complex - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
recombinant human  (R&D Systems)
94
R&D Systems recombinant human
Figure 6. Potential roles of the DNA-mediated proteolytic processing of HMGB1 by neutrophil elastase in NETs. Due to the enhanced binding activities of the processed HMGB1 protein, this processing may promote (1) <t>TLR4</t> signaling, (2) binding to biofilm DNA, and (3) DNA sensing by cGAS. Due to the loss of residues 177–215, the processing of HMGB1 may diminish (4) RAGE signaling and (5) nuclear localization. NET, neutrophil extracellular trap.
Recombinant Human, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human/product/R&D Systems
Average 94 stars, based on 1 article reviews
recombinant human - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
anti human md 2 antibody  (R&D Systems)
90
R&D Systems anti human md 2 antibody
Figure 6. Potential roles of the DNA-mediated proteolytic processing of HMGB1 by neutrophil elastase in NETs. Due to the enhanced binding activities of the processed HMGB1 protein, this processing may promote (1) <t>TLR4</t> signaling, (2) binding to biofilm DNA, and (3) DNA sensing by cGAS. Due to the loss of residues 177–215, the processing of HMGB1 may diminish (4) RAGE signaling and (5) nuclear localization. NET, neutrophil extracellular trap.
Anti Human Md 2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human md 2 antibody/product/R&D Systems
Average 90 stars, based on 1 article reviews
anti human md 2 antibody - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
recombinant human tlr4 md 2 complexes  (R&D Systems)
94
R&D Systems recombinant human tlr4 md 2 complexes
Figure 6. Potential roles of the DNA-mediated proteolytic processing of HMGB1 by neutrophil elastase in NETs. Due to the enhanced binding activities of the processed HMGB1 protein, this processing may promote (1) <t>TLR4</t> signaling, (2) binding to biofilm DNA, and (3) DNA sensing by cGAS. Due to the loss of residues 177–215, the processing of HMGB1 may diminish (4) RAGE signaling and (5) nuclear localization. NET, neutrophil extracellular trap.
Recombinant Human Tlr4 Md 2 Complexes, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human tlr4 md 2 complexes/product/R&D Systems
Average 94 stars, based on 1 article reviews
recombinant human tlr4 md 2 complexes - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
human tlr4 md2 proteins  (R&D Systems)
93
R&D Systems human tlr4 md2 proteins
Figure 6. Potential roles of the DNA-mediated proteolytic processing of HMGB1 by neutrophil elastase in NETs. Due to the enhanced binding activities of the processed HMGB1 protein, this processing may promote (1) <t>TLR4</t> signaling, (2) binding to biofilm DNA, and (3) DNA sensing by cGAS. Due to the loss of residues 177–215, the processing of HMGB1 may diminish (4) RAGE signaling and (5) nuclear localization. NET, neutrophil extracellular trap.
Human Tlr4 Md2 Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human tlr4 md2 proteins/product/R&D Systems
Average 93 stars, based on 1 article reviews
human tlr4 md2 proteins - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
anti mousemd2 polyclonal antibody  (Novus Biologicals)
90
Novus Biologicals anti mousemd2 polyclonal antibody
Figure 6. Potential roles of the DNA-mediated proteolytic processing of HMGB1 by neutrophil elastase in NETs. Due to the enhanced binding activities of the processed HMGB1 protein, this processing may promote (1) <t>TLR4</t> signaling, (2) binding to biofilm DNA, and (3) DNA sensing by cGAS. Due to the loss of residues 177–215, the processing of HMGB1 may diminish (4) RAGE signaling and (5) nuclear localization. NET, neutrophil extracellular trap.
Anti Mousemd2 Polyclonal Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mousemd2 polyclonal antibody/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
anti mousemd2 polyclonal antibody - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
md2  (Addgene inc)
93
Addgene inc md2
Figure 2 The CnB–TLR4 interaction results in cytokine production through IκB-α phosphorylation. (a), Quantitative PCR for NF-κB (2.1-fold) and IκB-α (9.3- fold). Po0.001, N = 3. (b) Western blot to detect IκB-α (with or without phosphorylation) and IL-8 in CnB-treated (lane 2) or buffer solution-treated (lane 1) U937 cells; the cells were stimulated with CnB or its buffer solution for 3 h. (c) Western blot to detect IκB-α phosphorylation at various time points in CnB- treated U937 cells. (d) Western blot to detect IκB-α phosphorylation in U937 cells treated with different antibodies. (e and f) ELISA for IL-8 and TNF-α in HEK293 cells transfected with TLR4 and/or <t>MD2</t> and treated with CnB and LPS for 3 h. The error bars represent the mean ± s.d.
Md2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/md2/product/Addgene inc
Average 93 stars, based on 1 article reviews
md2 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
anti md 2 antibody  (R&D Systems)
91
R&D Systems anti md 2 antibody
Figure 2 The CnB–TLR4 interaction results in cytokine production through IκB-α phosphorylation. (a), Quantitative PCR for NF-κB (2.1-fold) and IκB-α (9.3- fold). Po0.001, N = 3. (b) Western blot to detect IκB-α (with or without phosphorylation) and IL-8 in CnB-treated (lane 2) or buffer solution-treated (lane 1) U937 cells; the cells were stimulated with CnB or its buffer solution for 3 h. (c) Western blot to detect IκB-α phosphorylation at various time points in CnB- treated U937 cells. (d) Western blot to detect IκB-α phosphorylation in U937 cells treated with different antibodies. (e and f) ELISA for IL-8 and TNF-α in HEK293 cells transfected with TLR4 and/or <t>MD2</t> and treated with CnB and LPS for 3 h. The error bars represent the mean ± s.d.
Anti Md 2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti md 2 antibody/product/R&D Systems
Average 91 stars, based on 1 article reviews
anti md 2 antibody - by Bioz Stars, 2026-04
91/100 stars
  Buy from Supplier
rabbit polyclonal antibody to md2  (Novus Biologicals)
91
Novus Biologicals rabbit polyclonal antibody to md2
Figure 2 The CnB–TLR4 interaction results in cytokine production through IκB-α phosphorylation. (a), Quantitative PCR for NF-κB (2.1-fold) and IκB-α (9.3- fold). Po0.001, N = 3. (b) Western blot to detect IκB-α (with or without phosphorylation) and IL-8 in CnB-treated (lane 2) or buffer solution-treated (lane 1) U937 cells; the cells were stimulated with CnB or its buffer solution for 3 h. (c) Western blot to detect IκB-α phosphorylation at various time points in CnB- treated U937 cells. (d) Western blot to detect IκB-α phosphorylation in U937 cells treated with different antibodies. (e and f) ELISA for IL-8 and TNF-α in HEK293 cells transfected with TLR4 and/or <t>MD2</t> and treated with CnB and LPS for 3 h. The error bars represent the mean ± s.d.
Rabbit Polyclonal Antibody To Md2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibody to md2/product/Novus Biologicals
Average 91 stars, based on 1 article reviews
rabbit polyclonal antibody to md2 - by Bioz Stars, 2026-04
91/100 stars
  Buy from Supplier
tlr4 antagonist  (Selleck Chemicals)
93
Selleck Chemicals tlr4 antagonist
Figure 2 The CnB–TLR4 interaction results in cytokine production through IκB-α phosphorylation. (a), Quantitative PCR for NF-κB (2.1-fold) and IκB-α (9.3- fold). Po0.001, N = 3. (b) Western blot to detect IκB-α (with or without phosphorylation) and IL-8 in CnB-treated (lane 2) or buffer solution-treated (lane 1) U937 cells; the cells were stimulated with CnB or its buffer solution for 3 h. (c) Western blot to detect IκB-α phosphorylation at various time points in CnB- treated U937 cells. (d) Western blot to detect IκB-α phosphorylation in U937 cells treated with different antibodies. (e and f) ELISA for IL-8 and TNF-α in HEK293 cells transfected with TLR4 and/or <t>MD2</t> and treated with CnB and LPS for 3 h. The error bars represent the mean ± s.d.
Tlr4 Antagonist, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tlr4 antagonist/product/Selleck Chemicals
Average 93 stars, based on 1 article reviews
tlr4 antagonist - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier

Image Search Results


Figure 6. Potential roles of the DNA-mediated proteolytic processing of HMGB1 by neutrophil elastase in NETs. Due to the enhanced binding activities of the processed HMGB1 protein, this processing may promote (1) TLR4 signaling, (2) binding to biofilm DNA, and (3) DNA sensing by cGAS. Due to the loss of residues 177–215, the processing of HMGB1 may diminish (4) RAGE signaling and (5) nuclear localization. NET, neutrophil extracellular trap.

Journal: The Journal of biological chemistry

Article Title: DNA-mediated proteolysis by neutrophil elastase enhances binding activities of the HMGB1 protein.

doi: 10.1016/j.jbc.2022.102577

Figure Lengend Snippet: Figure 6. Potential roles of the DNA-mediated proteolytic processing of HMGB1 by neutrophil elastase in NETs. Due to the enhanced binding activities of the processed HMGB1 protein, this processing may promote (1) TLR4 signaling, (2) binding to biofilm DNA, and (3) DNA sensing by cGAS. Due to the loss of residues 177–215, the processing of HMGB1 may diminish (4) RAGE signaling and (5) nuclear localization. NET, neutrophil extracellular trap.

Article Snippet: Lyophilized TLR4 MD-2 complex was purchased from R&D Systems (catalog no.: #3146-TM-050).

Techniques: Binding Assay

Figure 2 The CnB–TLR4 interaction results in cytokine production through IκB-α phosphorylation. (a), Quantitative PCR for NF-κB (2.1-fold) and IκB-α (9.3- fold). Po0.001, N = 3. (b) Western blot to detect IκB-α (with or without phosphorylation) and IL-8 in CnB-treated (lane 2) or buffer solution-treated (lane 1) U937 cells; the cells were stimulated with CnB or its buffer solution for 3 h. (c) Western blot to detect IκB-α phosphorylation at various time points in CnB- treated U937 cells. (d) Western blot to detect IκB-α phosphorylation in U937 cells treated with different antibodies. (e and f) ELISA for IL-8 and TNF-α in HEK293 cells transfected with TLR4 and/or MD2 and treated with CnB and LPS for 3 h. The error bars represent the mean ± s.d.

Journal: Immunology and cell biology

Article Title: Calcineurin B stimulates cytokine production through a CD14-independent Toll-like receptor 4 pathway.

doi: 10.1038/icb.2015.91

Figure Lengend Snippet: Figure 2 The CnB–TLR4 interaction results in cytokine production through IκB-α phosphorylation. (a), Quantitative PCR for NF-κB (2.1-fold) and IκB-α (9.3- fold). Po0.001, N = 3. (b) Western blot to detect IκB-α (with or without phosphorylation) and IL-8 in CnB-treated (lane 2) or buffer solution-treated (lane 1) U937 cells; the cells were stimulated with CnB or its buffer solution for 3 h. (c) Western blot to detect IκB-α phosphorylation at various time points in CnB- treated U937 cells. (d) Western blot to detect IκB-α phosphorylation in U937 cells treated with different antibodies. (e and f) ELISA for IL-8 and TNF-α in HEK293 cells transfected with TLR4 and/or MD2 and treated with CnB and LPS for 3 h. The error bars represent the mean ± s.d.

Article Snippet: A recombinant plasmid containing TLR4 and/or MD2 (Addgene, Cambridge, MA, USA) was transfected into HEK293s using the Lipofectamine 3000 Transfection Reagent (Invitrogen), and then selected with neomycin for 6 days.

Techniques: Phospho-proteomics, Real-time Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay, Transfection